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Image Search Results
Journal: bioRxiv
Article Title: BLOC1S1 control of vacuolar organelle fidelity modulates T H 2 cell immunity and allergy susceptibility
doi: 10.1101/2024.03.21.586144
Figure Lengend Snippet: (A) Representative immunoblots for STING, P-IκBα, IκBα, P-NF-κB p65, NF-κB p65, GATA3, P- STAT6, STAT6 and β-actin from CTRL and TKO CD4 + T cells following DMSO or H151 (500 nM) incubation for 48 hours. (B) Protein quantitation and ratio of STING/β-actin, P-IKBα/IKBα, P-NF-kB P65/NF-kB P65, GATA3/β-actin and P-STAT6/STAT6 by densitometry analysis (n=6-7 per group). (C) IL-4, IL-5 and IL-13 cytokine release in activated CD4 + T cells treated with either DMSO or H151 (500nM) (n=6-7 per group). (D) Representative immunoblots for STING, P-IκBα, IκBα, P-NF-κB p65, NF-kB p65, GATA3 and β-actin from CTRL and TKO CD4 + T cells incubated with negative control (N.C.) or with STING siRNA. (E) Protein quantitation and ratio of STING/β-actin, P-IκBα/IκBα, P-NF-κB P65/NF-κB P65 and GATA3/β-actin by densitometry analysis (n=6 per group). (F) IL-4, IL-5 and IL-13 cytokine release in activated CD4 + T cells incubated with either N.C. or STING siRNA (n=9-18 per group). (G) Schematic representation of proposed mechanistic pathway. Values represent mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 vs control mice by two-way ANOVA followed by the Tukey’s post hoc test or unpaired two-tailed student-t-test.
Article Snippet: The following inhibitors were added to the cells for 24 hours before harvesting: the NF-κB inhibitor JSH23 (2 μM, Tocris Bioscience),
Techniques: Western Blot, Incubation, Protein Quantitation, Negative Control, Control, Two Tailed Test